A Genetic Perspective on Eye Evolution: Gene Sharing, Convergence and Parallelism
© U.S. Government 2008
Received: 23 June 2008
Accepted: 21 August 2008
Published: 26 September 2008
Did the diversity of lens-containing eyes evolve from one ancestral eye (monophyletic evolution) or from multiple, independently derived eyes (polyphyletic evolution)? Monophyletic evolution would make diverse eyes homologous (inherited similarities from a common ancestor); polyphyletic evolution would make eyes homoplasious (independently acquired similarities). Historically, anatomical and developmental differences among eyes of different species favored homoplasy; however, recent molecular data indicating that all eyes employ a similar cascade of transcription factors (proteins regulating gene expression) for development have suggested homology. Comparative studies on invertebrates and vertebrates suggest that the use of common networks of developmental transcription factors may be due to parallel evolution, a form of homoplasy by independent recruitments of similar genes and transcriptional networks. Remarkably, the photoreceptors of lens-containing jellyfish eyes have ciliary photoreceptors, like vertebrate photoreceptors, and apparently employ a vertebrate phototransduction system (linked biochemical processes converting light into nervous electrical impulse), consistent with parallel evolution between jellyfish and vertebrate eyes. Finally, the major proteins conferring the lens optical properties—the crystallins—were recruited by a gene-sharing process (the addition of a new gene function without loss of the original function) from various stress proteins and common metabolic enzymes in different species by convergent mutations (derived independently, not related by common ancestry) in their promoters (gene regulatory sequences) leading to high lens expression. Thus, the data indicate that homology or homoplasy of diverse eyes depends upon the level of analysis.
The complex and specialized nature of eyes has challenged evolutionists ever since Darwin wrote famously in his treatise on the origin of species [(Darwin 1859) p. 186] that “to suppose that the eye, with all its inimitable contrivances for adjusting the focus for different distances, for admitting different amounts of light, and for the correction of spherical and chromatic aberration, could have been formed by natural selection, seems, I freely confess, absurd in the highest possible degree.” Nonetheless, Darwin went on to express confidence that eyes did indeed evolve, and the accessibility and widespread distribution of eyes throughout the animal kingdom have made them a favorable subject for evolutionary studies (Arendt 2003; Arendt and Wittbrodt 2001; Fernald 2006; Land and Fernald 1992; Land and Nilsson 2002). The question as to whether the eye evolved once very early in the history of animals and diversified thereafter (monophyletic evolution) or whether an eye evolved multiple times independently in various forms as evolution progressed (polyphyletic evolution) has led to much discussion.
Agreeing on a proper definition for an eye can be itself a confounding issue, especially when considering eye evolution. Clearly there are different considerations if an eye is defined as simply a photoreceptor that responds to light in some fashion or if an eye is defined as a complex organ used in vision. For the present discussion an eye will be defined as an organ that can distinguish the directionality of light and that therefore has a dark shielding pigment along with two or more photoreceptors. Indeed, Land and Nilsson (1992) require that eyes must have the ability to detect an image, no matter how crude, by simultaneously comparing light from different directions; that is, eyes must have spatial vision. Although it is not possible to determine at present, if ever, how many times a light-responsive cell (photoreceptor) combined independently with a dark-shielding cell or other structure to create a primitive eye spot, I review here some salient features that favor the idea that diverse eyes have evolved with a considerable degree of independence and have different levels of homology (Land and Nilsson 2002; Fernald 2004).
Are Eyes Monophyletic or Polyphyletic?
Historically, the major anatomical and developmental differences between complex lens-containing eyes of vertebrates and some invertebrates (i.e., squid or octopus) or between lens-containing and compound eyes consisting of hundreds of ommatidia (separate facets with photoreceptors) were taken to mean that eyes arose multiple times or polyphyletically. Polyphyletic eye evolution is consistent with the facts that the morphology of the photoreceptors (retinal cells containing the protein, opsin, which coverts light to an electrical signal allowing vision) and the phototransduction signaling cascades (the linked biochemical processes in photoreceptors converting light into a nerve impulse) of invertebrates and vertebrates are generally different (except see last section of this review): invertebrates have rhabdomeric (microvillar) photoreceptors and employ a phospholipase C-based phototransduction cascade, while vertebrates have ciliated photoreceptors and employ a phosphodiesterase-based phototransduction cascade (Fernald 2004, 2006; Land and Nilsson 2002; see Gregory, this issue for further discussion). Moreover, early studies suggested that photoreceptors evolved 40–60 times (Salvini-Plawen and Mayr 1977), although this idea was challenged by Eakin, who believed that all ciliated photoreceptors had a common ancestor (Eakin 1979). Nevertheless, Eakin supported two origins for photoreceptors, ciliary and rhabdomeric. Computer-assisted estimations that complex eyes could evolve relatively quickly (within half a million years) starting from a light-sensitive skin patch gave credibility to the notion that different eyes evolved independently numerous times (Nilsson and Pelger 1994). That eyes exist only in about one-third of the animal phyla also supports polyphyletic eye evolution since there is no obvious reason why so many species would lose eyes and dispense with the selective advantage of vision.
In contrast to multiple origins of eyes, the common function of vision and the conserved use of opsin family members for phototransduction, despite phototransduction signaling differences in invertebrates and vertebrates, seemed consistent with the possibility that a primitive eyespot originated once and diversified thereafter during evolution (monophyletic evolution; see Gregory in this issue for further discussion). The recent influx of molecular data showing that a similar (although not necessarily identical) developmental regulatory network is employed for development of diverse eyes supported the notion that eyes are linked by common ancestry (Gehring 2005; see below). Indeed, reexamination of eye evolution in terms of developmental mechanisms (i.e., an evolutionary developmental biology, or evo–devo approach) greatly impacted the ideas of how diverse eyes originated.
Homology (Divergence) and Homoplasy (Parallelism and Convergence)
Gould has written an extensive book on evolution which reviews the historical basis and present understanding of homology, homoplasy, parallelism, and convergence (Gould 2002; see especially Chapter 10). Although Gould’s discussion is beyond the scope of the present article, it is worthwhile highlighting a few points to help understand the complexity of eye evolution. In brief, homology is similarity by inheritance from a common ancestor, and homoplasy is similarity by independent evolution. However, Gould points out that these concepts can become muddled and overlap by the fact that homoplasy can be divided into two processes: parallelism and convergence. Parallelism involves independent recruitment of similar, conserved regulatory processes, while convergence involves independent evolution of analogous functions using different regulatory processes. Gould states the following (p. 1074): “If we decide that the crucial distinction between homology and homoplasy should rest upon common ancestry vs. independent origin, then one important phenomenon, necessarily included within homoplasy by the defining criterion of independent origin for similar structures, shares too much conceptual overlap with homology to permit a clear and comfortable theoretical separation (however firm the descriptive division): independent origin channeled by common internal constraints of homologous genes or developmental pathways—in other words, the phenomenon known as parallelism.” Even though parallelism involves independent origin and homology-inherited evolution, these distinct evolutionary concepts can become intertwined, as expressed by Gould (p. 1079): “At the level of an overt phenotypic structure under explicit consideration, parallelism denies homology and asserts independent origin. But, at the level of the generators for the overt feature—the genes regulating its architecture and the developmental pathways defining its construction—parallelism affirms homology as the concept’s fundamental meaning and raison d’etre, and the basis for its dichotomous contrast with convergence as alternatives within the more inclusive category of homoplasy. Thus, parallelism does require independent regimes of similar selection, but the resulting phenotypic likenesses must also be channeled from within by homologous generators.” Homology as a descriptor of inherited relatedness, then, can be applied to different levels of biological or developmental organization. Gould distinguishes parallelism from convergence as follows (p. 1075): “...parallelism marks the formal influence of internal constraint, while convergence reflects the functional operation upon two substrates different enough to exclude internal factors as influences upon the resulting similarity.” In other words, the developmental mechanics of the evolution of a structure by parallelism or by convergence are different.
Gould’s descriptions help us appreciate the complexities of resolving the extent to which diverse eyes are monophyletic or polyphyletic, or stated otherwise, the extent to which diverse eyes evolved by the ancient eye scenario (monophyletic evolution) and by the parallel recruitment and convergent scenarios (polyphyletic evolution; Abouheif et al. 1997; Hodin 2000). For eyes to be monophyletic would require that each species inherited its eye developmental network (ancient eye scenario) rather than having independently recruited a similar developmental network (parallel recruitment scenario) or an entirely different developmental network (convergent scenario). The first alternative would make diverse eyes homologous (common descent from an ancestral form), the second alternative would make diverse eyes homoplasious by being independently derived in their lineages despite the utilization of similar developmental networks, and the third alternative would make diverse eyes homoplasious by being independently derived in their lineages using different developmental networks. The distinctions between these different aspects of evolution are not always obvious and can be a matter of degree, especially if one considers developmental potential that is not always utilized. Indeed, it has been suggested (Abouheif 2008) that parallelism may be considered a special and distinct phase of evolution characterized by “the flickering on or off of characters between closely related species through time.” This special phase encompassing patterns of parallel evolution using distinct mechanisms has been called “mesoevolution” (Abouheif 2008).
As discussed below, the presence of diverse eyes cannot be understood in terms of any one of these evolutionary processes by itself. Eye evolution involves homology, parallelism and convergence, depending on the level being considered.
Similar Transcriptional Networks Have Been Used for Eye Development During Evolution
A turning point away from a polyphyletic view of eye evolution based on the marked differences in eye anatomy towards a monophyletic view came with the demonstration that the gene encoding the developmental transcription factor Pax6 (called Eyeless in Drosophila) is essential for eye development in flies (Quiring et al. 1994) and humans (Ton et al. 1991; Glaser et al. 1992; Hanson 2003) and can even induce eyes in anatomical regions that do not normally have eyes (such as legs, antennae, or wings) in flies (Halder et al. 1995; Tomarev et al. 1997) and vertebrates (Chow et al. 1999). Recent studies show that these extra supernumerary eyes (often called ectopic eyes) in strange places respond to light and may be functional in the sense that they extend nerve projections to the central nervous system (Clements et al. 2008). Comparative studies throughout the animal kingdom suggested that Pax6 is a “master gene” for eye development (Gehring 2004; Gehring and Ikeo 1999). In addition, a plethora of investigations showed that the Pax6 protein consistently works within a similar group of proteins (called a developmental cascade or network of transcription factors) that directs eye development by regulating the expression of many different genes. The conserved developmental cascade of transcription factors regulating gene expression is known as the Pax/Six/Eya/Dach regulatory gene network for eye development; it is also called the retinal determination gene network (Silver and Rebay 2005; Kozmik 2008). The repeated employment of a similar developmental cascade of transcription factors for eye development during evolution reinforced the notion that an ancestral eyespot was constructed once and was subsequently modified during evolution in different species, producing the diversity of eyes seen today, and could be interpreted as the ancient eye scenario, namely, monophyletic eye evolution by inheritance from a common ancestor
The idea that all eyes are homologous because that they use Pax6 within a similar developmental cascade, or network, of transcription factors is compelling; however, it is not without difficulties (Simpson and Price 2002; Van Heyningen and Williamson 2002). First, there are species that have members of the eye developmental cascade but do not have eyes. The nematode Cenorhabitis elegans is among the best-studied eyeless species, where Pax6 is used for head and sensory neuron development (Chisholm and Horvitz 1995; Zhang and Emmons 1995). There are also cases in which eyes develop without Pax6. These include the adult eyes of the polychaete annelid worm (Platynereis; Arendt et al. 2002), the Hesse eyecup of the cephalochordate amphioxus (Branchiostoma; Glardon et al. 1998), and eye regeneration in the planaria flatworms (Pineda et al. 2002). In addition, the hierarchy of the transcription factors used in the cascade may differ within the network; moreover, the “master gene” concept is complicated by the fact that other proteins within the cascade can induce ectopic eyes (Silver and Rebay 2005; Kumar and Moses 2001a; Pichaud et al. 2001) or lenses (Oliver and Gruss 1997). The different transcription factors comprising the developmental cascade function with different degrees of importance during eye development in different species, making some members of the cascade critical for eye development in one species but not in another species. An example is the eyes absent (eya) gene which is essential for eye development in flies, but its role in eye development in mice is much less clear [see (Donner and Maas 2004) for review].
Other questions arise when considering homology on the basis of the conserved use of a similar cascade of transcription factors for the development of diverse eyes. A general issue is that gene regulatory networks have been referred to as “tool kits” because of their promiscuous use in multiple tissues (Conway Morris 2003). For example, the critical transcription factor Pax6, for eye development, is also used for the development of the brain, nose, and pancreas in mice (Dahl et al. 1997), and the Pax/Six/Eya/Dach regulatory gene network, or at least members of the gene families used in this network, direct not only eye development but also that of many other tissues depending upon the species (Silver and Rebay 2005; Kozmik 2008; Donner and Maas 2004; Relaix and Buckingham 1999; Heanue et al. 1999; Kawakami et al. 2000; Kardon et al. 2004; Rebay et al. 2005; Kozmik et al. 2007). However, biology is complex, and more information is required in order to establish homology by the use of similar developmental cascades of transcription factors. Different combinations of the members of a developmental cascade may lead to significant differences in its function, and gene regulatory networks respond to various signaling cues and may contain different members of duplicated genes that are closely related but not identical (see Silver and Rebay 2005; Kumar and Moses 2001b; Zuber et al. 2003; Schlosser 2006). In addition, changes in the genetic makeup of the regulatory networks used for eye development in different species can occur during evolution leading to diversification between eyes sharing a common ancestor. In this connection, it has been proposed that developmental pathways are modified by a process called intercalary evolution (Gehring and Ikeo 1999). Intercalary evolution can occur by gene duplication and divergence, by recruitment of novel genes into the pathway via changes in gene regulation, or by various forms of evolutionary tinkering.
Thus, a type of monophyletic eye evolution based on the conservation of a gene regulatory cascade for eye development cannot be refuted at the present time. Additional data in the future should provide new insights into the evolutionary significance of the common use of similar genetic networks for eye development in different species.
Lens and Crystallins: The Concept of Gene Sharing
Complex eyes contain transparent cellular lenses just behind (posterior to) the cornea. Lenses differ in shape, relative size, and hardness in different species of invertebrates and vertebrates in accordance with the different mechanisms used for focusing (accommodation) and improve vision by increasing refractive power while allowing more light into the eye (Land and Nilsson 2002; Jonasova and Kozmik 2008). Lenses accumulate globular, water-soluble cytoplasmic proteins called crystallins. Crystallins are named as such because they are present in the crystal-clear lens. Crystallins form a refractive index gradient in the lens, highest in the center and lowest at the periphery which shortens the focal length of the transmitted light and eliminates spherical and chromatic aberrations. Hard spherical lenses dominate the refractive power in eyes of aquatic species because there is a negligible difference in the refractive index of the surface cornea and the surrounding water. In the environment of nonaquatic species, where the refractive index between the cornea and air is large, the cornea located at the surface of the eye provides about two-thirds and the internal lens about one-third of the refractive power of the eye.
Taxon-specific crystallins are often (not always) common metabolic enzymes (known as enzyme crystallins) that are expressed at lower levels in many tissues where they have nonrefractive roles. Even the crystallins that are present in all vertebrate eyes (i.e., the α-crystallins and the β/γ-crystallins) and would seem to be highly specialized for their structural lens role in vision are also expressed in other tissues where they have non-optical roles. For example, the α-crystallins are stress-inducible small heat shock proteins (Ingolia and Craig 1982; Klemenz et al. 1991; Horwitz 1992), and if not for their accumulation in the lens where they have a refractive function, they would be classified strictly as members of the small heat shock protein family. We have called this dual role for an identical protein of refraction in the lens and catalysis or stress protection within or outside of the lens, “gene sharing” (Piatigorsky et al. 1988; Piatigorsky and Wistow 1989; Piatigorsky 1992). In other words, a crystallin gene encodes a protein with more than one biochemical or molecular function. Thus, gene sharing expands the functional significance of a gene, which clearly has evolutionary implications.
Gene sharing among crystallins provides a striking example of how tissue homology can be hierarchical. Lenses of different species may have elements of homology as judged by using similar inherited genetic networks to direct their development; yet, they acquired independently a variety of different crystallins for their optical roles. In short, there is no one answer to the question of whether eyes or lenses are homologous or not: it depends on the level of analysis.
Recruiting Unrelated Crystallin Genes by Convergent Evolution
Both the multiple functions and the taxon-specificity of crystallins were totally unexpected in view of the specialized nature and overall similarity in structure and function of lenses in different species. Apart from all crystallins being water-soluble, their defining characteristic is their abundance in the lens, which is a requirement to affect the refractive index of the transparent tissue. What, then, might account for the diversity of lens crystallins?
One method of accumulating a protein in a tissue is to have its gene very active in that tissue. Gene activity is controlled by specific, short DNA sequences (called cis-control elements) in the promoter and enhancer regions associated with the gene. Promoters are situated just in front of the protein-coding region of the gene, while enhancers can be in front of, within, or even behind the gene. In addition to controlling the intensity of gene activity (i.e., the amount of messenger RNA made by the gene), promoters and enhancers also determine the tissues in which the gene is expressed by binding different combinations of transcription factors. Indeed, this is the molecular basis of how the specific genetic cascades discussed above function, namely the transcription factor members of the cascade sequentially bind the promoters and enhancers of different genes that must be activated to direct eye development. Expression of a gene in a particular tissue, then, requires that certain transcription factors present in that tissue bind to and activate the promoter and enhancer of that gene.
The diverse crystallins are thus unified less by the nature of their proteins than by the similarities in the regulation of their genes, which was achieved by independent evolution of gene regulatory sequences in promoters and enhancers. The sequence changes recruiting or modifying regulatory elements did not necessarily extinguish the original expression patterns of their associated genes in other tissues. Many of the recruited crystallin genes continued their original expression patterns and functions in addition to serving as lens crystallin genes, fulfilling the criterion for gene sharing of serving more than one molecular function.
In conclusion of this section, it is important to underline that the recruitment of nonhomologous crystallin genes occurred by independent changes in their promoters and enhancers, resulting in their gaining affinity for transcription factors that are members of the conserved developmental network used for eye and lens development in all species that have been studied. Crystallin evolution, thus, involved a combination of homologous and convergent processes.
Evidence for Parallel Eye Evolution in Cnidarians and Vertebrates
The lenses of T. cystophora have three distinct crystallins (J1, J2, and J3), and each crystallin polypeptide is encoded in a separate gene. The J1 crystallins comprise three extremely similar polypeptides, while J2 and J3 crystallins are each a single polypeptide (Piatigorsky et al. 1989, 1993, 2001; Kozmik et al. 2008b). The jellyfish crystallins are unrelated to crystallins of other species consistent with their being recruited as crystallins independently in jellyfish. The crystallin genes are expressed in a number of jellyfish tissues, suggesting that they have non-lens functions and have evolved by a gene-sharing process as have crystallins of other species. The putative non-lens functions of the jellyfish crystallins are not known. J1 crystallins belong to a subfamily of proteins that show similarity to ADP-ribosylation enzymes (Castellano et al. 2005) and J3 crystallin shows similarity to saposins, which are multifunctional proteins involved in membrane turnover (Piatigorsky et al. 2001). J2 crystallin is a novel protein of unknown function that has not been found in other species (Kozmik et al. 2008b). The transparent lenses of jellyfish eyes, then, have high refractive power associated with a gradient of unique, independently recruited crystallins.
Unlike other species which activate their crystallin promoters with Pax6 (see Fig. 5), jellyfish lack Pax6 and have, instead, PaxB (Kozmik 2005). Pax6 and Pax2 (also involved in aspects of eye development) are believed to have been derived by gene duplication of a common ancestor to PaxB. PaxB, not Pax6, binds and activates the promoters of the jellyfish crystallin genes (Kozmik et al. 2003, 2008b). Jellyfish PaxB does not activate expression of crystallin genes of other species. It follows that jellyfish have independently recruited unique lens crystallins using a Pax transcription factor that does not exist in other species with lens-containing eyes.
Comparison of jellyfish and vertebrate retinas suggest that they evolved in parallel by independently recruiting similar genes in their retinas. First, the photoreceptors of the invertebrate jellyfish more closely resemble the vertebrate ciliary type than the invertebrate rhabdomeric type (Wray 2007; Kozmik et al. 2008a; Eakin and Westfall 1962). Moreover, multiple opsins from different species of cnidarians (the phylum in which jellyfish are situated) more closely resemble those of vertebrates than those of invertebrates (Suga et al. 2008). In addition, there is molecular biological evidence that the jellyfish photoreceptors employ a vertebrate-like phototransduction system (Kozmik et al. 2008a). Further studies, however, are required to establish the precise similarities and differences between jellyfish and vertebrate phototransduction processes.
Another similarity between jellyfish and vertebrate eyes involves the dark shielding pigment providing visual orientation [see (Kozmik et al. 2008a) for data, further discussion and references]. Invertebrates use a pigment comprising pterins and/or ommochromes (a polychaete annelid, Platynereis dumerilii and Drosophila) or rarely melanin (the planarian, Dugesia). Vertebrates use exclusively melanin as the dark-shielding pigment of the retina. It appears that jellyfish use melanin as do vertebrates for its dark-shielding eye pigment. Interestingly, the jellyfish melanin resides within the photoreceptors rather than in separate pigmented cells, as is the case in vertebrates. It is not known whether the incorporation of the pigment granules into the jellyfish photoreceptors reflects an ancestral or derived state.
Invertebrates and Vertebrates Both Have Ciliary and Rhabdomeric Photoreceptors and Opsins
Although beyond the scope of the present article, but as a point of interest in the fascinating story of eye evolution, it turns out that invertebrate and vertebrate eyes are not as markedly split into two categories defined by having either ciliary or rhabdomeric photoreceptors or opsins as implied above. The polychaete annelid worm P. dumerilii has rhabdomeric photoreceptors with r-opsin in the eye and ciliary photoreceptors with c-opsin in the brain (Arendt et al. 2004). These findings were interpreted as follows: a species developed an ancestral eyespot with undefined ancestral photoreceptors and opsin which underwent cell and gene duplications to give rise to rhabdomeric and ciliated photoreceptors with r-opsin and c-opsin, respectively. The rhabdomeric photoreceptors were used for vision in the retina, and the ciliated photoreceptors were used for brain activity during evolution in invertebrates. Vertebrate evolution involved a transition: the invertebrate brain ciliary photoreceptors were incorporated into the retina for vision and the invertebrate retinal rhabdomeric photoreceptors transformed into the vertebrate retinal ganglion cells, which contain the r-opsin related melanopsin and were used for photoperiodicity (Foster and Hankins 2007).
Taken together, it appears that both parallel and convergent evolutionary processes played a role in eye evolution. The diverse lens crystallins comprise a variety of unrelated multifunctional proteins serving refractive functions in the lens and nonoptical functions (often enzymatic or stress protective functions) in other tissues utilizing a process called gene sharing. The recruitment and high lens expression of crystallins were accomplished during evolution by the independent acquisition of mutations in the cis-control elements of the promoters and enhancers of their genes, making the crystallin genes responsive to a similar set of transcription factors used for eye development. Thus, the diverse lens crystallins were recruited by convergent changes in the regulation of unrelated genes.
In contrast to the striking diversity of lens crystallins, the photoreceptors and the phototransduction cascades in jellyfish and vertebrates appear unexpectedly similar. Although these similarities do not prove that jellyfish and vertebrates evolved eyes in parallel by independently recruiting related genes, it is difficult to explain the similarities of these disparate species by strict inheritance, as would be required for homology in view of the differences in phototransduction between vertebrates and invertebrates.
How such a remarkable common independent gene recruitment process characteristic of parallel evolution may have taken place remains conjectural. A promising hypothesis is that parallel evolution involving similar pathways was forced by constraints that limit the options that could have been implemented (Hodin 2000; Abouheif 2008). In that connection, Kozmik (2005) has put forth a bipartite model for eye evolution based on the DNA-binding paired domain and homeodomain of Pax proteins. The case is made that the crucial role of the paired domain for eye morphogenesis, pigment cell development, and crystallin gene expression, and the importance of the homeodomain for opsin synthesis are coupled in one molecule, the Pax transcription factor. In Kozmik’s words, “…the morphological unity found in the eye, a photoreceptor linked to the shading pigment, is mirrored on the molecular level, by uniting two independent DNA-binding domains in one regulatory protein.” Such a constraint would make it difficult to dissociate Pax proteins and their associated transcription factors from eye development during evolution.
Finally, a personal comment: Science often has a narrative character that rivals any great piece of literature. The scientific narratives are based on real characters (molecules) that have been studied and represented to the best of the scientist’s ability. The plots are created by the experimental paths seeking answers to the questions posed by the scientist. Clearly different routes (sets of experiments) can be followed, and the data (language, imagery) can be expressed in numerous ways. No two scientists will develop identical narratives just as no two authors will write identical novels, although both of the authors or both of the scientists could address similar issues and have similar outcomes. The multiple aspects of eye evolution—gene sharing, convergence, homology, parallelism—all occurring simultaneously reflect the enormous richness of choices that Nature provides at each instant over hundreds of millions of years under changing conditions. The ambiguities in evolutionary history that we spectators-at-a-distance are left with resemble the literature qualities of science where the great conflicts are seldom resolved to finality. We are writing narratives about characters we never met that were born and in some cases died during events before our time, and that may have had similar or quite different functions during their lifetime than their progeny have today. These ambiguities are not like those of The Trial by Kafka, where one never learns what crime was committed but more like historical narratives where one knows the major actors, the plot, and the results yet still argues about the precise nature of the characters or the details of the environment or reported events, all of which may significantly impact the final conclusions. The ambiguities of evolutionary history in no way deny that there was such a history, but they provide space for new perspectives that can modify the narrative. We know the characters of the diverse extant eyes, we reconstruct their histories the best we can, but we are never certain to what extent and when similar properties were inherited or derived independently. It may have some value, then, to keep in mind that science, like literature, is a human endeavor holding great truths and many possibilities.
I thank Drs. Stanislav Tomarev for critically reading the manuscript and Zbynek Kozmik for many productive discussions on the topics of this review.
- Abouheif E. Parallelism as the pattern and process of mesoevolution. Evol Dev 2008;10:3–5.View ArticlePubMedGoogle Scholar
- Abouheif E, Akam M, Dickinson WJ, Holland PW, Meyer A, Patel NH, et al. Homology and developmental genes. Trends Genet 1997;13:432–3. doi:10.1016/S0168-9525(97)01271-7.View ArticlePubMedGoogle Scholar
- Arendt D. Evolution of eyes and photoreceptor cell types. Int J Dev Biol 2003;47:563–71.PubMedGoogle Scholar
- Arendt D, Wittbrodt J. Reconstructing the eyes of Urbilateria. Philos Trans R Soc Lond B Biol Sci 2001;356:1545–63. doi:10.1098/rstb.2001.0971.View ArticlePubMedGoogle Scholar
- Arendt D, Tessmar K, de Campos-Baptista MI, Dorresteijn A, Wittbrodt J. Development of pigment-cup eyes in the polychaete Platynereis dumerilii and evolutionary conservation of larval eyes in Bilateria. Development 2002;129:1143–54.PubMedGoogle Scholar
- Arendt D, Tessmar-Raible K, Snyman H, Dorresteijn AW, Wittbrodt J. Ciliary photoreceptors with a vertebrate-type opsin in an invertebrate brain. Science 2004;306:869–71. doi:10.1126/science.1099955.View ArticlePubMedGoogle Scholar
- Carosa E, Kozmik Z, Rall JE, Piatigorsky J. Structure and expression of the scallop Omega-crystallin gene. Evidence for convergent evolution of promoter sequences. J Biol Chem 2002;277:656–64. doi:10.1074/jbc.M107004200.View ArticlePubMedGoogle Scholar
- Castellano S, Lobanov AV, Chapple C, Novoselov SV, Albrecht M, Hua D, et al. Diversity and functional plasticity of eukaryotic selenoproteins: identification and characterization of the SelJ family. Proc Natl Acad Sci USA 2005;102:16188–93. doi:10.1073/pnas.0505146102.View ArticlePubMedGoogle Scholar
- Chepelinsky AB, King CR, Zelenka PS, Piatigorsky J. Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5′ flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia. Proc Natl Acad Sci U S A 1985;82:2334–8. doi:10.1073/pnas.82.8.2334.View ArticlePubMedGoogle Scholar
- Chisholm AD, Horvitz HR. Patterning of the Caenorhabditis elegans head region by the Pax-6 family member vab-3. Nature 1995;377:52–5. doi:10.1038/377052a0.View ArticlePubMedGoogle Scholar
- Chow RL, Altmann CR, Lang RA, Hemmati-Brivanlou A. Pax6 induces ectopic eyes in a vertebrate. Development 1999;126:4213–22.PubMedGoogle Scholar
- Clements J, Lu Z, Gehring WJ, Meinertzhagen IA, Callaerts P. Central projections of photoreceptor axons originating from ectopic eyes in Drosophila. Proc Natl Acad Sci U S A 2008;105:8968–73. doi:10.1073/pnas.0803254105.View ArticlePubMedGoogle Scholar
- Coates MM. Visual ecology and functional morphology of Cubuzoa (Cnidaria). Integr Comp Biol 2003;43:542–8. doi:10.1093/icb/43.4.542.View ArticlePubMedGoogle Scholar
- Coates MM, Garm A, Theobald JC, Thompson SH, Nilsson DE. The spectral sensitivity of the lens eyes of a box jellyfish, Tripedalia cystophora (Conant). J Exp Biol 2006;209:3758–65. doi:10.1242/jeb.02431.View ArticlePubMedGoogle Scholar
- Conway Morris S. Life's solutions. Inevitable humans in a lonely universe. Cambridge: Cambridge University Press; 2003.View ArticleGoogle Scholar
- Cvekl A, Piatigorsky J. Lens development and crystallin gene expression: many roles for Pax-6. Bioessays 1996;18:621–30. doi:10.1002/bies.950180805.View ArticlePubMedGoogle Scholar
- Cvekl A, Sax CM, Bresnick EH, Piatigorsky J. A complex array of positive and negative elements regulates the chicken alpha A-crystallin gene: involvement of Pax-6, USF, CREB and/or CREM, and AP-1 proteins. Mol Cell Biol 1994;14:7363–76.View ArticlePubMedGoogle Scholar
- Cvekl A, Kashanchi F, Sax CM, Brady JN, Piatigorsky J. Transcriptional regulation of the mouse alpha A-crystallin gene: activation dependent on a cyclic AMP-responsive element (DE1/CRE) and a Pax-6-binding site. Mol Cell Biol 1995;15:653–60.View ArticlePubMedGoogle Scholar
- Cvekl A, Yang Y, Chauhan BK, Cveklova K. Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens. Int J Dev Biol 2004;48:829–44. doi:10.1387/ijdb.041866ac.View ArticlePubMedGoogle Scholar
- Dahl E, Koseki H, Balling R. Pax genes and organogenesis. Bioessays 1997;19:755–65. doi:10.1002/bies.950190905.View ArticlePubMedGoogle Scholar
- Darwin C. On the origin of species by means of natural selection, or preservation of favored races in the struggle for life. London: Murray; 1859.Google Scholar
- de Jong WW, Hendriks W, Mulders JW, Bloemendal H. Evolution of eye lens crystallins: the stress connection. Trends Biochem Sci 1989;14:365–8. doi:10.1016/0968-0004(89)90009-1.View ArticlePubMedGoogle Scholar
- Donner AL, Maas RL. Conservation and non-conservation of genetic pathways in eye specification. Int J Dev Biol 2004;48:743–53. doi:10.1387/ijdb.041877ad.View ArticlePubMedGoogle Scholar
- Eakin R. Evolutionary significance of photoreceptors: in retrospect. Am Zool 1979;19:647–53.View ArticleGoogle Scholar
- Eakin RM, Westfall JA. Fine structrue of photoreceptors in the hydromedusan, Polyorchis penicillatus. Proc Natl Acad Sci U S A 1962;48:826–33.View ArticlePubMedGoogle Scholar
- Ekstrom P, Garm A, Palsson J, Vihtelic TS, Nilsson DE. Immunohistochemical evidence for multiple photosystems in box jellyfish. Cell Tissue Res 2008;333:115–24. doi:10.1007/s00441-008-0614-8.View ArticlePubMedGoogle Scholar
- Fernald RD. Evolving eyes. Int J Dev Biol 2004;48:701–5. doi:10.1387/ijdb.041888rf.View ArticlePubMedGoogle Scholar
- Fernald RD. Casting a genetic light on the evolution of eyes. Science 2006;313:1914–8. doi:10.1126/science.1127889.View ArticlePubMedGoogle Scholar
- Fitch WM. Homology a personal view on some of the problems. Trends Genet 2000;16:227–31. doi:10.1016/S0168-9525(00)02005-9.View ArticlePubMedGoogle Scholar
- Foster RG, Hankins MW. Circadian vision. Curr Biol 2007;17:R746–51. doi:10.1016/j.cub.2007.07.007.View ArticlePubMedGoogle Scholar
- Garm A, Bielecki J. Swim pacemakers in box jellyfish are modulated by the visual input. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2008;194:641–51.View ArticlePubMedGoogle Scholar
- Garm A, Ekstrom P, Boudes M, Nilsson DE. Rhopalia are integrated parts of the central nervous system in box jellyfish. Cell Tissue Res 2006;325:333–43. doi:10.1007/s00441-005-0134-8.View ArticlePubMedGoogle Scholar
- Garm A, O'Connor M, Parkefelt L, Nilsson DE. Visually guided obstacle avoidance in the box jellyfish Tripedalia cystophora and Chiropsella bronzie. J Exp Biol 2007a;210:3616–23. doi:10.1242/jeb.004044.View ArticlePubMedGoogle Scholar
- Garm A, Coates MM, Gad R, Seymour J, Nilsson DE. The lens eyes of the box jellyfish Tripedalia cystophora and Chiropsalmus sp. are slow and color-blind. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2007b;193:547–57.View ArticlePubMedGoogle Scholar
- Gehring WJ. Historical perspective on the development and evolution of eyes and photoreceptors. Int J Dev Biol 2004;48:707–17. doi:10.1387/ijdb.041900wg.View ArticlePubMedGoogle Scholar
- Gehring WJ. New perspectives on eye development and the evolution of eyes and photoreceptors. J Hered 2005;96:171–84. doi:10.1093/jhered/esi027.View ArticlePubMedGoogle Scholar
- Gehring WJ, Ikeo K. Pax 6: mastering eye morphogenesis and eye evolution. Trends Genet 1999;15:371–7. doi:10.1016/S0168-9525(99)01776-X.View ArticlePubMedGoogle Scholar
- Glardon S, Holland LZ, Gehring WJ, Holland ND. Isolation and developmental expression of the amphioxus Pax-6 gene (AmphiPax-6): insights into eye and photoreceptor evolution. Development 1998;125:2701–10.PubMedGoogle Scholar
- Glaser T, Walton DS, Maas RL. Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene. Nat Genet 1992;2:232–9. doi:10.1038/ng1192-232.View ArticlePubMedGoogle Scholar
- Gould SJ. The structure of evolutionary theory. Cambridge: Harvard University Press; 2002. p. 1433.Google Scholar
- Halder G, Callaerts P, Gehring WJ. Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila. Science 1995;267:1788–92. doi:10.1126/science.7892602.View ArticlePubMedGoogle Scholar
- Hanson IM. PAX6 and congenital eye malformations. Pediatr Res 2003;54:791–6. doi:10.1203/01.PDR.0000096455.00657.98.View ArticlePubMedGoogle Scholar
- Heanue TA, Reshef R, Davis RJ, Mardon G, Oliver G, Tomarev S, et al. Synergistic regulation of vertebrate muscle development by Dach2, Eya2, and Six1, homologs of genes required for Drosophila eye formation. Genes Dev 1999;13:3231–43. doi:10.1101/gad.13.24.3231.View ArticlePubMedGoogle Scholar
- Hodin J. Plasticity and constraints in development and evolution. J Exp Zool 2000;288:1–20. doi: 10.1002/(SICI)1097-010X(20000415)288:1<1::AID-JEZ1>3.0.CO;2-7.View ArticlePubMedGoogle Scholar
- Horwitz J. Alpha-crystallin can function as a molecular chaperone. Proc Natl Acad Sci U S A 1992;89:10449–53. doi:10.1073/pnas.89.21.10449.View ArticlePubMedGoogle Scholar
- Hughes AL. The evolution of functionally novel proteins after gene duplication. Proc R Soc Lond B Biol Sci 1994;256:119–24. doi:10.1098/rspb.1994.0058.View ArticleGoogle Scholar
- Hughes AL. Adaptive evolution of genes and genomes. New York: Oxford Universtity Press; 1999.Google Scholar
- Hughes AL. Gene duplication and the origin of novel proteins. Proc Natl Acad Sci U S A 2005;102:8791–2. doi:10.1073/pnas.0503922102.View ArticlePubMedGoogle Scholar
- Ingolia TD, Craig EA. Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin. Proc Natl Acad Sci U S A 1982;79:2360–4. doi:10.1073/pnas.79.7.2360.View ArticlePubMedGoogle Scholar
- Jonasova K, Kozmik Z. Eye evolution: lens and cornea as an upgrade of animal visual system. Semin Cell Dev Biol 2008;19:71–81. doi:10.1016/j.semcdb.2007.10.005.View ArticlePubMedGoogle Scholar
- Kardon G, Heanue TA, Tabin CJ. The Pax/Six/Eya/Dach network in development and evolution. In: Scholosser G, Wagner GP, editors. Modularity in development and evolution. Chicago: The University of Chicago Press; 2004. p. 59–80.Google Scholar
- Kawakami K, Sato S, Ozaki H, Ikeda K. Six family genes—structure and function as transcription factors and their roles in development. Bioessays 2000;22:616–26. doi:10.1002/1521-1878(200007)22:7<616::AID-BIES4>3.0.CO;2-R.View ArticlePubMedGoogle Scholar
- Kimura M, Ota T. On some principles governing molecular evolution. Proc Natl Acad Sci USA 1974;71:2848–52. doi:10.1073/pnas.71.7.2848.View ArticlePubMedGoogle Scholar
- Klement JF, Wawrousek EF, Piatigorsky J. Tissue-specific expression of the chicken alpha A-crystallin gene in cultured lens epithelia and transgenic mice. J Biol Chem 1989;264:19837–44.PubMedGoogle Scholar
- Klemenz R, Frohli E, Steiger RH, Schafer R, Aoyama A. Alpha B-crystallin is a small heat shock protein. Proc Natl Acad Sci USA 1991;88:3652–6. doi:10.1073/pnas.88.9.3652.View ArticlePubMedGoogle Scholar
- Kondoh H, Katoh K, Takahashi Y, Fujisawa H, Yokoyama M, Kimura S, et al. Specific expression of the chicken delta-crystallin gene in the lens and the pyramidal neurons of the piriform cortex in transgenic mice. Dev Biol 1987;120:177–85. doi:10.1016/0012-1606(87)90116-3.View ArticlePubMedGoogle Scholar
- Kozmik Z. Pax genes in eye development and evolution. Curr Opin Genet Dev 2005;15:430–8. doi:10.1016/j.gde.2005.05.001.View ArticlePubMedGoogle Scholar
- Kozmik Z. The role of Pax genes in eye evolution. Brain Res Bull 2008;75:335–9. doi:10.1016/j.brainresbull.2007.10.046.View ArticlePubMedGoogle Scholar
- Kozmik Z, Daube M, Frei E, Norman B, Kos L, Dishaw LJ, et al. Role of Pax genes in eye evolution. A Cnidarian PaxB gene uniting Pax2 and Pax6 functions. Dev Cell 2003;5:773–85. doi:10.1016/S1534-5807(03)00325-3.View ArticlePubMedGoogle Scholar
- Kozmik Z, Holland ND, Kreslova J, Oliveri D, Schubert M, Jonasova K, et al. Pax-Six-Eya-Dach network during amphioxus development: conservation in vitro but context specificity in vivo. Dev Biol 2007;306:143–59. doi:10.1016/j.ydbio.2007.03.009.View ArticlePubMedGoogle Scholar
- Kozmik Z, Ruzickova J, Jonasova K, Matsumoto Y, Vopalensky P, Kozmikova I, et al. Assembly of the cnidarian camera-type eye from vertebrate-like components. Proc Natl Acad Sci U S A 2008a;105:8989–93. doi:10.1073/pnas.0800388105.View ArticlePubMedGoogle Scholar
- Kozmik Z, Swamynathan SK, Ruzickova J, Jonasova K, Paces V, Vlcek C, et al. Cubozoan crystallins: evidence for convergent evolution of pax regulatory sequences. Evol Dev 2008b;10:52–61.View ArticlePubMedGoogle Scholar
- Kumar JP, Moses K. Eye specification in Drosophila: perspectives and implications. Semin Cell Dev Biol 2001a;12:469–74. doi:10.1006/scdb.2001.0270.View ArticlePubMedGoogle Scholar
- Kumar JP, Moses K. EGF receptor and Notch signaling act upstream of Eyeless/Pax6 to control eye specification. Cell 2001b;104:687–97. doi:10.1016/S0092-8674(01)00265-3.View ArticlePubMedGoogle Scholar
- Land MF, Fernald RD. The evolution of eyes. Annu Rev Neurosci 1992;15:1–29. doi:10.1146/annurev.ne.15.030192.000245.View ArticlePubMedGoogle Scholar
- Land MF, Nilsson DE. Animal eyes. Oxford: Oxford University Press; 2002.Google Scholar
- Laska VG, Hundgen M. Morphologie und ultrastruktur der lichtsinnesorgane von Tridpedalia cystophora Conant (Cnidaria, Cubozoa). Zool Jb Anat 1982;108:107–23.Google Scholar
- Nilsson DE, Pelger S. A pessimistic estimate of the time required for an eye to evolve. Proc R Soc Lond B Biol Sci 1994;256:53–8. doi:10.1098/rspb.1994.0048.View ArticleGoogle Scholar
- Nilsson DE, Gislen L, Coates MM, Skogh C, Garm A. Advanced optics in a jellyfish eye. Nature 2005;435:201–5. doi:10.1038/nature03484.View ArticlePubMedGoogle Scholar
- Ohno S. Evolution by gene duplication. New York: Springer; 1970.View ArticleGoogle Scholar
- Oliver G, Gruss P. Current views on eye development. Trends Neurosci 1997;20:415–21. doi:10.1016/S0166-2236(97)01082-5.View ArticlePubMedGoogle Scholar
- Piatigorsky J. Lens crystallins and their genes: diversity and tissue-specific expression. FASEB J 1989;3:1933–40.PubMedGoogle Scholar
- Piatigorsky J. Lens crystallins. Innovation associated with changes in gene regulation. J Biol Chem 1992;267:4277–80.PubMedGoogle Scholar
- Piatigorsky J. Gene sharing and evolution: the diversity of protein functions. Cambridge: Harvard University Press; 2007.View ArticleGoogle Scholar
- Piatigorsky J, Kozmik Z. Cubozoan jellyfish: an Evo/Devo model for eyes and other sensory systems. Int J Dev Biol 2004;48:719–29. doi:10.1387/ijdb.041851jp.View ArticlePubMedGoogle Scholar
- Piatigorsky J, Wistow GJ. Enzyme/crystallins: gene sharing as an evolutionary strategy. Cell 1989;57:197–9. doi:10.1016/0092-8674(89)90956-2.View ArticlePubMedGoogle Scholar
- Piatigorsky J, Wistow G. The recruitment of crystallins: new functions precede gene duplication. Science 1991;252:1078–9. doi:10.1126/science.252.5009.1078.View ArticlePubMedGoogle Scholar
- Piatigorsky J, O'Brien WE, Norman BL, Kalumuck K, Wistow GJ, Borras T, et al. Gene sharing by delta-crystallin and argininosuccinate lyase. Proc Natl Acad Sci U S A 1988;85:3479–83. doi:10.1073/pnas.85.10.3479.View ArticlePubMedGoogle Scholar
- Piatigorsky J, Horwitz J, Kuwabara T, Cutress CE. The cellular eye lens and crystallins of cubomedusan jellyfish. J Comp Physiol [A] 1989;164:577–87. doi:10.1007/BF00614500.View ArticleGoogle Scholar
- Piatigorsky J, Horwitz J, Norman BL. J1-crystallins of the cubomedusan jellyfish lens constitute a novel family encoded in at least three intronless genes. J Biol Chem 1993;268:11894–901.PubMedGoogle Scholar
- Piatigorsky J, Norman B, Dishaw LJ, Kos L, Horwitz J, Steinbach PJ, et al. J3-crystallin of the jellyfish lens: similarity to saposins. Proc Natl Acad Sci U S A 2001;98:12362–7. doi:10.1073/pnas.231310698.View ArticlePubMedGoogle Scholar
- Pichaud F, Treisman J, Desplan C. Reinventing a common strategy for patterning the eye. Cell 2001;105:9–12. doi:10.1016/S0092-8674(01)00292-6.View ArticlePubMedGoogle Scholar
- Pineda D, Rossi L, Batistoni R, Salvetti A, Marsal M, Gremigni V, et al. The genetic network of prototypic planarian eye regeneration is Pax6 independent. Development 2002;129:1423–34.PubMedGoogle Scholar
- Quiring R, Walldorf U, Kloter U, Gehring WJ. Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans. Science 1994;265:785–9. doi:10.1126/science.7914031.View ArticlePubMedGoogle Scholar
- Rebay I, Silver SJ, Tootle TL. New vision from Eyes absent: transcription factors as enzymes. Trends Genet. 2005;21:163–71. doi:10.1016/j.tig.2005.01.005.View ArticlePubMedGoogle Scholar
- Relaix F, Buckingham M. From insect eye to vertebrate muscle: redeployment of a regulatory network. Genes Dev 1999;13:3171–8. doi:10.1101/gad.13.24.3171.View ArticlePubMedGoogle Scholar
- Salvini-Plawen LV, Mayr E. On the evolution of photoreceptors and eyes. Evol Dev 1977;10:207–63.Google Scholar
- Schlosser G. Induction and specification of cranial placodes. Dev Biol 2006;294:303–51. doi:10.1016/j.ydbio.2006.03.009.View ArticlePubMedGoogle Scholar
- Sheng G, Thouvenot E, Schmucker D, Wilson DS, Desplan C. Direct regulation of rhodopsin 1 by Pax-6/eyeless in Drosophila: evidence for a conserved function in photoreceptors. Genes Dev 1997;11:1122–31. doi:10.1101/gad.11.9.1122.View ArticlePubMedGoogle Scholar
- Silver SJ, Rebay I. Signaling circuitries in development: insights from the retinal determination gene network. Development 2005;132:3–13. doi:10.1242/dev.01539.View ArticlePubMedGoogle Scholar
- Simpson TI, Price DJ. Pax6; a pleiotropic player in development. Bioessays 2002;24:1041–51. doi:10.1002/bies.10174.View ArticlePubMedGoogle Scholar
- Suga H, Schmid V, Gehring WJ. Evolution and functional diversity of jellyfish opsins. Curr Biol 2008;18:51–5. doi:10.1016/j.cub.2007.11.059.View ArticlePubMedGoogle Scholar
- Taylor JS, Raes J. Duplication and divergence: the evolution of new genes and old ideas. Annu Rev Genet 2004;38:615–43. doi:10.1146/annurev.genet.38.072902.092831.View ArticlePubMedGoogle Scholar
- Tomarev SI, Callaerts P, Kos L, Zinovieva R, Halder G, Gehring W, et al. Squid Pax-6 and eye development. Proc Natl Acad Sci U S A 1997;94:2421–6. doi:10.1073/pnas.94.6.2421.View ArticlePubMedGoogle Scholar
- Ton CC, Hirvonen H, Miwa H, Weil MM, Monaghan P, Jordan T, et al. Positional cloning and characterization of a paired box- and homeobox-containing gene from the aniridia region. Cell 1991;67:1059–74. doi:10.1016/0092-8674(91)90284-6.View ArticlePubMedGoogle Scholar
- van Heyningen V, Williamson KA. PAX6 in sensory development. Hum Mol Genet 2002;11:1161–7. doi:10.1093/hmg/11.10.1161.View ArticlePubMedGoogle Scholar
- Wistow GJ, Piatigorsky J. Lens crystallins: the evolution and expression of proteins for a highly specialized tissue. Annu Rev Biochem 1988;57:479–504. doi:10.1146/annurev.bi.57.070188.002403.View ArticlePubMedGoogle Scholar
- Wray GA. The evolutionary significance of cis-regulatory mutations. Nat Rev Genet 2007;8:206–16. doi:10.1038/nrg2063.View ArticlePubMedGoogle Scholar
- Zhang Y, Emmons SW. Specification of sense-organ identity by a Caenorhabditis elegans Pax-6 homologue. Nature 1995;377:55–9. doi:10.1038/377055a0.View ArticlePubMedGoogle Scholar
- Zuber ME, Gestri G, Viczian AS, Barsacchi G, Harris WA. Specification of the vertebrate eye by a network of eye field transcription factors. Development 2003;130:5155–67. doi:10.1242/dev.00723.View ArticlePubMedGoogle Scholar