Predator Escape: An Ecologically Realistic Scenario for the Evolutionary Origins of Multicellularity
© Pentz et al. 2015
Received: 22 March 2015
Accepted: 30 July 2015
Published: 11 August 2015
The origin of multicellularity was transformative for life on earth, allowing for the evolution of the large, complex organisms we see today. Despite its clear importance, the evolution of multicellularity remains a challenging topic to teach, especially at the high school level. Here we present a quick (one or two 50-min sessions) and easy laboratory in which students experimentally examine the hypothesis that small-mouthed predators can select for multicellularity. Students first observe rotifers and yeast (both unicellular and multicellular strains) separately under the microscope. Based on the observed natural history of the predator, they create a hypothesis for how predation will affect the survival of each type of yeast. They then test this hypothesis by feeding the yeast to the rotifers, quantifying the number of unicellular and multicellular yeast consumed, and analyze this result statistically. Pre- and post-lab assessment demonstrates the efficacy of our hands-on curricula in teaching fundamental concepts about multicellularity, as well as evolution in general.
The evolution of complex life on Earth has occurred through key steps in which formerly autonomous organisms evolve to become integral parts of a larger, higher-level organism. Smith and Szathmary (1995) termed these the major transitions in evolution, and they include the origin of eukaryotic cells from an endosymbiosis, the evolution of multicellular organisms, and even the evolution of ‘superorganisms’ composed of many multicellular entities. Multicellular organisms have independently evolved from unicellular ancestors at least 25 times in the history of life (Grosberg and Strathmann 2007), resulting in the origin of paradigmatic complex macroorganisms like plants and animals. Despite its fundamental importance in biology, early steps in this transition remain poorly understood. This is largely because multicellularity arose a long time ago in Earth’s extant lineages (>200 million years ago) (Herron et al. 2009; Grosberg and Strathmann 2007), and early forms of multicellularity have been lost to extinction.
The transition to multicellularity involved a fundamental shift in the nature of biological organization (Buss 1987; Smith and Szathmary 1995). Single cells, formerly organisms in their own right, evolve to become parts in a new, larger individual (Buss 1987; Smith and Szathmary 1995). The first step in this transition was likely the evolution of simple multicellular clusters (Bonner 1998; Boraas et al. 1998; Kirk 2005; Pfeiffer and Bonhoeffer 2003). Once clusters evolve, the next step involves a shift in the level of selection, from single-cells to clusters (Damuth and Heisler 1988; Michod 2005). Here natural selection discriminates mainly between whole multicellular clusters, not between cells within them. If clusters possess the necessary ingredients for evolution via natural selection (that is, they possess heritable variation in multicellular traits and succeed or fail because of those traits), then between-cluster selection can result in the evolution of new cluster-level traits. This cluster-level adaptation can result in the evolution of increased multicellular complexity (Willensdorfer 2009; Bonner 2003).
Here we describe a novel laboratory experiment for high school and college students to study the very first step in the transition to multicellularity—the evolution of cellular clusters. Previous work has suggested that the advantages of increased size may have led to the evolution of multicellularity (Bonner 1965, 1998, 2000; King 2004). Proposed benefits include environmental protection (Gerhert and Kirschner 1997), cooperative metabolism (Dworkin 1972; Koschwanez et al. 2013), and immunity from predation by filter-feeding predators (Stanley 1973; Bonner 1988). Our research program uses the unicellular fungus baker’s yeast (Saccharomyces cerevisiae) to study the evolution of multicellularity. Rather than focus on a selection mechanism underlying one of the above described hypotheses to select for increased size, we use a simple property of fluid dynamics: clusters of cells settle faster through liquid than single cells. This has proven to be a remarkably effective method of evolving multicellularity. Within just 60 days, clusters of cells (termed ‘snowflake yeast’) evolved and displaced their single-celled ancestors in all ten replicate populations studied (Ratcliff et al. 2012). We have previously described a student lab, modeled on the experiment of Ratcliff et al. (2012), in which students experimentally evolve multicellular yeast de novo (Ratcliff et al. 2014). Although this lab allows students to critically think about the evolution of multicellularity, it faces two significant challenges. First, experimental evolution requires substantial investments in both time (2 weeks of daily transfers) and resources (hundreds of tubes of sterile media), and few classes can work it into their schedules. Second, our selective agent (rapid settling though liquid media) was not chosen because we think that settling selection led to multicellularity in nature—we use it because it is a rapid and reliable method of selecting for larger size. Here we present a lab that circumvents both of these limitations, allowing students to examine how selection imposed by small-mouthed predators can favor the evolution of simple multicellularity.
In this lab, students examine the ability for small-mouthed predators to select for simple multicellularity by assaying the survival of uni- and multicellular yeast that are fed to rotifers. In the first exercise, students observe the yeast (both uni- and multicellular) and rotifer separately, and formulate a prediction on if the rotifers will preferentially consume uni- or multicellular yeast. In the second exercise, students study the natural history of this interaction, examining how rotifers catch prey, and examining how both uni- and multicellular yeast fare in the face of predation. In the third exercise, students quantify the relative survival of uni- and multicellular yeast during predation, then analyze their results statistically.
The list of materials required for this lab can be found on page 2 of the teacher’s guide (Additional file 1). The snowflake yeast kit can be obtained free of charge by emailing Prof. Will Ratcliff (firstname.lastname@example.org). Rotifers can be ordered from Carolina Biological Supply Company (Item #133172, genus Philodina). We have included everything else required for this lab in the supplementary material: a student version of the lab manual (Additional file 2), two introductory PowerPoint presentations (Additional files 3, 4), videos of rotifer predation (Additional files 5, 6), pictures for quantifying rotifer predation (Additional file 7), and a pre-lab/post-lab test to assess student learning (Additional file 8). We intend to update this lab periodically, so please check our website (www.snowflakeyeastlab.com) for the latest curricular materials.
The Prey and The Predator
Exercise 1: Predicting Rotifer Predation
To begin the lab, students start out by simply observing the rotifers and yeast separately. 100 µL of Philodina rotifers are placed onto a depression slide and viewed on a compound microscope. Rotifers should be sucked up from the bottom of the container, as this is where they are the most concentrated. Students should pay special attention how the rotifers feed. Next, students view the yeast (included in the free kit) on the microscope by first diluting each stock culture 1:10 in water, then placing 5 µL of each diluted culture on a standard slide. At this point, students create both a prediction and a hypothesis for how multicellular yeast should fare when fed to the rotifers relative to the unicellular strain. We believe that this is good practice, given how frequently predictions and hypotheses are conflated by students. If instructors only have one 50-min lab session to perform this lab, this entire observational section (Exercise 1) can be skipped to save time.
Exercise 2: Observing Rotifer Predation
Next, students feed the yeast to the rotifers to observe the effect of rotifer predation on the survival of uni- and multicellular strains. If the students have observed the rotifers and yeast separately (above), 5 µL of each yeast culture can be added directly to the depression slide containing the rotifers, once again shaking the capped tubes vigorously to homogenize the yeast culture prior to use. Alternatively, students will add 100 µL of Philodina rotifers and 5 µL of each yeast culture together onto a depression slide. At this point, a coverslip can be added to the depression slide and the slide should be viewed immediately on a compound microscope.
Once the slide is on the microscope, students will observe the rotifers as they eat. Students should observe at least 10 feeding rotifers and examine which yeast is preyed upon more readily (Fig. 1c). Questions they can answer include: How do the rotifers eat? How long does it take for a rotifer to fill its stomach? What type of yeast (either uni- or multicellular) do the rotifers appear to prefer? Why might this be?
Exercise 3: Quantifying Rotifer Predation
If the value obtained by the students is greater than 3.84, then the result is significant at p < 0.05, indicating that preference exhibited by the rotifers is statistically robust. While this equation might look a little daunting, we have found that most 9th grade biology students have little problem with it.
The above lab allows students to examine how predators can select for cellular clusters, the first step in the evolutionary transition to multicellularity. Observant students might point out that the lab has a chicken-and-egg problem: the rotifer predators are themselves a multicellular animal, so predation by a rotifer-like predator couldn’t have led to the origin of the first multicellular organisms. We use rotifers in this experiment because they are large, slow moving, transparent (allowing observation of gut contents), and easy to handle. While they are not as easy to work with, unicellular predators (such as Paramecium aurelia) may be used instead of (or in addition to) rotifers. P. aurelia can be obtained from Carolina Biological Supply Company (Item #131546). These predators exhibit many differences in their physiology and behavior (the most relevant being their nearly continuous and rapid movement, which makes microscopy difficult), but still strongly prefer to consume unicellular yeast. Like the rotifers, red and blue yeast cells can be counted in flattened P. aurelia, allowing students to statistically analyze feeding preference using eqs. 1 and 2. For a full description of how to use Paramecium in this lab, see Appendix 1 in Additional file 1.
Statistical analysis of pre-lab and post-lab results
Mann–Whitney U p value
Fraction bootstrapped runs in which post-lab > pre-lab (%)
“I think the unicellular yeast would start to evolve into multicellularity. This would happen as the multicellular yeast thrived while the unicellular yeast was suffering… Only the large rotifers with larger mouths (who could eat multicellular yeast) would be able to thrive. Natural selection would act on them and soon they would evolve to be larger and have larger mouths”.
This student, a freshman in her first biology class, was able to figure out the concept of a co-evolutionary arms race from context of the lab and prompt alone.
The major transitions in evolution, and macroevolution more generally, remain challenging topics to teach, especially in introductory biology classes. Indeed, the process through which radically new forms of life arise through Darwinian processes is hardly intuitive. This laboratory allows students to make the connection between macroevolutionary changes in morphology and the microevolutionary processes (e.g., mutation, selection, etc.) that underlie them. One hurdle that has long faced teachers trying to incorporate macroevolution into their curricula is the difficulty of exploring the subject with hands-on, organism-centric labs. Most labs exploring macroevolutionary change have relied on comparisons with living lineages, fossils, or computer simulations (Dubowsky and Hartman 1986; Rodrígues et al. Rodríguez et al. 2006; Soderberg and Price 2003). While these are valuable teaching materials, inquiry based exercises that utilize living organisms can be more transparent, and offer a direct connection to the ecological theater in which evolutionary processes play out (Delpech 2009; Green et al. 2011; Olson and Loucks-Horsley 2000; Plunkett and Yampolsky 2010).
The lab described here incorporates hands-on exercises to teach key concepts about the evolution of multicellularity. In just two 50-min lab periods, students are able to examine how predation can lead to cluster formation, the first step in the transition to multicellularity. This is one of the central hypotheses for how multicellularity may have arisen in extant multicellular lineages (Bonner 1998). The lab incorporates observation and natural history, hypothesis formulation, collection of quantitative data, and statistical analysis, immersing students in the practice of science. In addition to teaching students about the ecological conditions that may have led to the origin of novel multicellular organisms, this lab teaches basic concepts about (1) Darwinian evolution, (2) the multilevel selection hypothesis for the evolutionary transition to multicellularity, (3) cellular division of labor, (4) benefits and costs of multicellularity, (5) and the history of multicellularity on Earth.
This lab addresses a number of Core Ideas and cross-cutting concepts described in the Next Generation Science Standards (Schweingruber et al. 2012; see the NGSS page on our website for a more detailed discussion: http://www.snowflakeyeastlab.com/standards.htm). Core Idea LS1 “hinges on the unifying principle that cells are the basic unit of life.” At its foundation, this lab is about the central role of cells in biology, as seen through the lens of the evolutionary transition to multicellularity. Core Idea LS2 examines the ecological interaction of organisms with their environment, including “how social interactions and group behavior play out within and between species”. This lab addresses these issues directly, examining how predation, a between-species social interaction, can produce a selective pressure favoring group formation in yeast. While this lab does not allow students to examine evolution directly (for this see our experimental evolution lab module, Ratcliff et al. 2014), the lecture, readings, and discussion questions focus on evolutionary history and process. As a result, this lab teaches students about core ideas from LS3 (Heredity) and LS4 (Biological Evolution). Specifically, students will be taught about how microevolutionary processes can precipitate macroevolutionary change by applying Darwin’s logic to cellular clusters (i.e., if clusters possess heritable genetic variation for multicellular traits, then selection acting on the properties of clusters can result in cluster-level adaptation). Finally, this lab emphasizes three cross-cutting concepts. First, students examine cause and effect through careful crafting and quantitative testing of a hypothesis for how multicellularity affects susceptibility to rotifer predation. Second, it teaches students to use model systems to explore big questions, and third, allows students to make connections between structure and function.
The bottom line: this lab is a fun, inexpensive, quick, and effective way to teach both high school and university students about macroevolution and the origin of biological complexity.
Description of the Supplementary Files
We have provided several resources to help teachers run this lab. Teacher and student guides contain a detailed overview of the lab with step-by-step instructions for students (Additional files 1, 2). We provide two well-annotated introductory PowerPoint presentations for use introducing key background material on multicellularity and the lab activity, the first of which is detailed and may be more suited for university classes and AP biology (Additional file 3) while the second is a simpler version that may be better suited for some high school classes (Additional file 4). We provide two videos detailing rotifer predation (Additional files 5, 6). For schools with limited access to high quality microscopes, we include a set of images of flattened rotifers that can be used to quantitate rotifer predation preference in exercise 3 (Additional file 7). Finally, the pre-lab and post-lab assessment are included in Additional file 8.
WCR, TL, and NB conceived of and designed the lab. JTP and WCR analyzed the data. JTP wrote the first draft of the paper (all authors, especially WCR, provided constructive feedback). All authors read and approved the final manuscript.
This work was supported by the National Science Foundation grant no. NSF/DEB1051115 AM3.
Compliance with Ethical Guidelines
Competing Interests The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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